本书针对非线性光学及其共聚焦显微技术介绍了相关的理论、技术以及应用前景。书中对不同非线性光学显微镜成像技术做了比较和综述，而且在合适的部分介绍了其理论基础，理论部分和技术紧密结合，并且通过介绍理论计算手段使读者能够将理论基础快速应用到实际工作中。
本书主要面向凝聚态物理、光学以及生物学相关领域的科研人员、学者、研究生与高年级本科生，可作为相关领域科学研究的参考资料。

Chapter 1 Introduction
Chapter 2 Basic Theory of Nonlinear Optics
2.1 Classical electromagnetic theory of nonlinear optics
2.1.1 Measurement of nonlinear optical processes
2.1.2 Nonlinearly induced polarization effect of optical media
2.1.3 Tensor representation of nonlinear polarization
2.1.4 Rotational symmetry of nonlinear polarizability tensor elements
2.1.5 Time reversal symmetry of polarization rate
2.2 Quantum theory and method of nonlinear optics
2.2.1 Density matrix
2.2.2 Timedependent density matrix
2.2.3 The tensor and properties of the polarizability of the independent molecular system
2.2.4 The tensor and properties of the polarizability of the molecular system with intermolecular interaction
2.2.5 Resonanceenhanced polarizability
2.2.6 Calculation method of nonlinear polarizability by higher order derivative
2.2.7 Nonlinear polarizability by sum over state method
2.3 Common nonlinear optical processes
2.3.1 Second harmonic generation
2.3.2 Sum frequency generation
2.3.3 Raman amplification
2.3.4 Fourwave mixing
Chapter 3 The Principle, Application and Imaging of CARS
3.1 Principles of CARS
3.1.1 Mechanism of CARS signal generation
3.1.2 CARS optical configuration
3.2 Biomedical imaging of CARS
3.3 Materials imaging of CARS
3.3.1 CARS image for porous carbon
3.3.2 CARS image for graphene
Chapter 4 The Principle, Application and Imaging of SRS
4.1 Principles of SRS
4.1.1 Quantum theory of SRS
4.1.2 Instrumentation of SRS
4.2 Biomedical imaging
4.2.1 SRS imaging of hela cells
4.2.2 SRS detection and diagnosis of the boundary of glioma
4.2.3 SRS imaging of laryngeal cancer
4.3 Material composition analysis
Chapter 5 The Principle, Application and Imaging of SHG
5.1 Principles of SHG
5.2 Biomedical imaging of SHG
5.2.1 Collagen
5.2.2 SHG imaging for elastic arteries
5.2.3 SHG imaging for snail
Chapter 6 The Principle, Application and Imaging of TPEF
6.1 Principles of TPEF
6.1.1 Twophoton absorption
6.1.2 Design of strong TPA cross section
6.1.3 Twophoton excited/emitted fluoresence
6.1.4 TPEF optical configuration
6.2 Biomedical imaging of TPEF
6.2.1 TPEF and lifetime imaging for glioma
6.2.2 TPEF and lifetime imaging for gastrointestinal cancer
Chapter 7 The Principle, Application and Imaging of STED
7.1 Principles of STED
7.1.1 Selection of excitation and loss laser types
7.1.2 Selection of excitation and loss wavelengths
7.2 Biomedical imaging of STED
7.2.1 Nervous structure imaging
7.2.23 D STED imaging
Chapter 8 PlasmonEnhanced Nonlinear Spectroscopy and Imaging
8.1 Principles
8.1.1 Plasmon
8.1.2 Enhancement mechanism
8.2 Application of surface plasmon enhanced nonlinear optical signals
8.2.1 Surfaceplasmonenhanced CARS
8.2.2 Surfaceplasmonenhanced TPEF
8.2.3 Surfaceplasmonenhanced highorder harmonic wave generate
Bibliography
Acknowledgement
Indexes